Understanding Biological Indicators: Time-Lapse Video of Geobacillus stearothermophilus Growth

Nov 4 / Chris Maughan

Biological indicators (BIs) play a crucial role in ensuring that sterilization processes are effective. These indicators are typically spore-forming bacteria, which are particularly resistant to sterilization methods. One of the most commonly used organisms in autoclave validation and steam sterilization testing is Geobacillus stearothermophilus, a thermophilic bacterium chosen for its resistance to high temperatures.

Today, we invite you to take a closer look at the biological indicator incubation process. In this post, we’re excited to share a time-lapse video showing the growth of Geobacillus stearothermophilus ampoule type biological indicator.

Why Use Biological Indicators?

Biological indicators are essential in validating sterilization processes across industries such as pharmaceuticals, healthcare, and biotechnology. Unlike chemical indicators, BIs provide a direct measure of sterilization efficacy by confirming the destruction of highly resistant microorganisms. 

Time Lapse Video: Geobacillus stearothermophilus Growth in Action

Our video captures the growth of Geobacillus stearothermophilus spores in a ampoule, showing a positive result. Alongside a negative control biological indicator. Both ampoules were placed inside a 55-60c Incubator.
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Ensure Confidence in your Sterilization Process

At Thermal Compliance Ltd, we specialize in autoclave validation and sterility assurance, working with clients to achieve the highest standards of compliance. By partnering with our expert consultancy team, you can ensure your sterilization processes meet regulatory requirements and perform as expected in real-world conditions.

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Join us on one of our 'Advanced Autoclave Validation' courses for more validation insights.
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